Composite

Part:BBa_K2217024:Design

Designed by: Mohammad Tarek Mansour   Group: iGEM17_AFCM-Egypt   (2017-09-25)


CMV Enhancer + CMV Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Part BBa_K2217022 was composed of CMV Enhancer as well as CMV Promoter (BBa_K2217000 and BBa_K2217006 respectively) as constitutive promoters to enhance the transcription process of the laci that was submitted at (BBa_K2217012).

More information on the design of this part can be found at [http://2017.igem.org/Design.html# Egypt-AFCM Design]


Source

Synthesized Fragments by IDT

References

[1] Romanienko PJ, Giacalone J, Ingenito J, et al. A Vector with a Single Promoter for In Vitro Transcription and Mammalian Cell Expression of CRISPR gRNAs. Fujii H, ed. PLoS ONE. 2016;11(2):e014836.